Probeliatm PCR system for rapid detection of Salmonella in milk powder and ricotta cheese
نویسندگان
چکیده
منابع مشابه
Selective Amplification of prt, tyv and invA Genes by Multiplex PCR for Rapid Detection of Salmonella typhi
A multiplex PCR-based assay was developed for detection of Salmonella typhi and identification of other salmonella serotypes. Three primer-sets were selected from different genomic sequences, malo2-F/malo2-Ra primers from invasion gene, Parat-s/Parat-as as well as tyv-s/tyv-as primers from O-antigen gene cluster of the genus Salmonella. This method differentiated Salmonella spp., based on size ...
متن کاملDetection of Coxiella burnetii by Real-Time PCR in Raw Milk and Traditional Cheese Distributed in Tehran Province
Coxiella burnetii is common causative agent of Q fever in humans and animals. Although the main route of human infection is through inhalation of contaminated aerosols (dust), but oral transmission through contaminated raw milk and dairy products are also possible routes of infection. The aim of this study was to identify and determine the extent of contamination and prevalence of Coxiella burn...
متن کاملDetermination of RT-PCR detection limit of live and dead Salmonella cells in raw and sterilized milk
The objective of the current study was to evaluate the reproducibility of a reverse transcriptase PCR (RT-PCR)-based technique to differentiate viable and dead Salmonella cells in raw and sterilized milk. The microorganism was initially inoculated into the milk samples followed by incubating at 37°C for 4 h prior to inactivation by heat at 80°C for 10 min. The treated and non-treated samples we...
متن کاملrapid and specific detection of salmonella typhimurium by pcr-elisa
objectives: salmonella typhimurium is important food-borne pathogen responsible for gastroenteritis. in this work a polymerase chain reaction based enzyme linked immunosorbent assay (pcr-elisa) was developed to identify salmonella typhimurium. materials and methods: the rfb gene which is responsible for biosynthesis of the salmonella o-antigenic lipopolysaccharide was selected as the target s...
متن کاملA Multi-Omics Approach to Evaluate the Quality of Milk Whey Used in Ricotta Cheese Production
In the past, milk whey was only a by-product of cheese production, but currently, it has a high commercial value for use in the food industries. However, the regulation of whey management (i.e., storage and hygienic properties) has not been updated, and as a consequence, its microbiological quality is very challenging for food safety. The Next Generation Sequencing (NGS) technique was applied t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2000
ISSN: 0266-8254,1365-2673
DOI: 10.1046/j.1472-765x.2000.00723.x